Introduction: It has been shown that ambient air pollution leads to increased cardiovascular mortality, and studies found associations between ambient air pollution and hospital admissions for various cardiovascular diseases. In this study, the effects of air pollutants on cardiac arrhythmia and heart rate variability (HRV) were evaluated and the mechanism of arrhythmogenesis was elucidated. Materials and methods: we will use the ambient PM exposure collected using portable mini volume sampler during morning (AM 7:00 ~ AM 10). This particle matters was instillated via trachea to isoproterenol induced myocardiac injury rat medel. Twenty-four hours after exposure, the animals are lightly anesthetized and 3 ml of blood will be collected by cardiac puncture. Echocardiography was performed for evaluation of cardiac function. Ex-vivo ventricular arrhythmia induction was performed by electrical stimulator. Optical mapping was performed. Telemetry monitoring was recorded during all procedure and HRV (heart rate variability) was acquired. Results: There were no significant difference between control and PM instillation group in Ventricular tachyarrhythmia(VT/VF) occurrence: 3.1짹1.2 times in control group vs 2.5짹0.6 times in PM group, p=0.3; There were significant difference in HRV parameters (mean NN, SDNN, SDANN, RMSSD, p=0.03). In optical mapping, there were significant difference of action potential duration at 70%(APD70) between control and PM group at normal(37.1짹32.2ms vs 31.0짹34.3ms), borderzone (59.7짹51.8ms vs 80.8짹48.7ms) and infarction area(89.4짹47.4ms vs 122.8짹24.0ms) respectively(p=0.86 , p=0.04 and p=0.03). there was no significant difference in infarcted myocardium(H-E stain; control vs PM, 29.1% vs 29.5%, p=0.85) Conclusion: ambient PM exposure affect the autonomic function and arrhythmogenesis in infracted heart without change of heart function or infarcted myocardiac size.