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ȣ - 510512 142 |
Statin Treatment Can Enhance The Homing of Mesenchymal Stem Cells Into Heart By Systemic Delivery in Rat Infarct Model |
전남대학교병원 심장센터¹, 전남대학교 심혈관질환 치료재생 사업단², 화순전남대학교병원 핵의학과³ |
박혜정, 안영근¹ ², 권진숙¹ ², 김용숙¹ ², 홍문화¹ ², 태성호³, 민정준³, 범희승³, 정명호¹ ², 조정관¹, 박종춘¹ |
Background: Bone marrow derived mesenchymal stem cell (BM-MSC) is an attractive source for myocardial repair. In vivo imaging using adenovirus encoding firefly luciferase gene (Ad-CMV-Fluc) is a method to trace the transplanted live cells. Recently, statins have been interested as a pleiotrophic effect on cardiovascular disease. We investigated the BM-MSCs engrafting rate by systemic delivery into heart according to the Zocor administration in rat infarct model.
Methods: Rats were received Zocor (1.67 mg/kg/day) by gastric gavage 1 day prior to ischemia-reperfusion (I/R) injury, then continued for 7 days. A similar amount of drinking water was given to the other two groups every day. I/R injury model was induced by ligation of LAD for 4 hours followed by relase and conducted BM-MSCs transplantation through tail vein injection containing 1x106 BM-MSCs being made to express Fluc reporter gene. At 7 days after BM-MSCs transplantation, optical bioluminescence imaging was then conducted to determine the degree of cell distribution rate in heart using a cooled charged-coupled device (CCD) camera (Xenogen). The rats were assigned to: (1) I/R+PBS, (2) I/R+BM-MSCs, (3) Zocor+I/R+BM-MSCs.
Results: Cardiac bioluminescence signals were determined at 7 days after transplantation; I/R+PBS (0 p/s/cm2/sr), I/R+BM-MSCs (3.75e+0.3 p/s/cm2/sr), Zocor+I/R+BM-MSCs (4.653e+0.3 p/s/cm2/sr). The rats that received Zocor displayed enhanced cardiac luminescence signals that that of only cell treated group.
Conclusion: Statin treatment may be an efficient tool for BM-MSCs transplantation by systemic delivery in ischemic heart disease. The more detailed informations including histologic analysis will be presented.
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