Effective gene delivery of potent angiogenic growth factor via highly efficient expression vector will improve angiogenesis in many ischemic diseases. Hepatocyte growth factor X7 gene(HGF X7) is the most potent isoform in releasing the hepatocyte growth factor in vivo and in vitro. We developed highly efficient expression vector, pCK, which can drive high level of angiogenic factor in mouse skeletal muscle. The aim of this study was to assess the angiogenic efficiency of new hepatocyte growth factor X7 gene via pCK vector in rabbit ischemic hindlimb model. Unilateral femoral artery was excised in rabbits and injected 500 microgram of pCK(n=11), pCK-HGF(n=10), and pCK-HGF X7(n=16) at femoral muscles after 10 days. After 40 days, angiography and Doppler study were performed. Angiography revealed more collateral vessels were developed in pCK-HGF and pCK-HGF X7 than pCK.(p<0.05) And capillary count from histology revealed more capillaries formation in pCK-HGF, pCK-HGF X7 than pCK.(p<0.05) Maximal blood flow was increased in pCK-HGF, pCK-HGF X7 than pCK.(p<0.05) In conclusion, pCK is efficient vector for gene delivery and pCK-HGF and pCK-HGF X7 are effective for new vessel formation and can achieve therapeutic benefits in rabbit hindlimb ischemia model and may be useful for peripheral artery disease.