Background: We produced a large animal model of acute myocardial infarction induced by transcatheeter injection of the left anterior descending artery (LAD) using a alcohol. Methods: Eight female pigs underwent thranscatheter injection of the distal LAD using alcohol to produce apicoanterior myocardial infarction. The low pressure ballooning was performed using 2.5mm over-the-wire balloon system at just below the second diagonal branch. We injected 1ml of the ethyl alcohol for 1 min and occlulded of the LAD using balloon for another 5 min. Follow-up coronary angiography and Left ventricular (LV) angiography was performed 4 weeks after alcohol injection and coronary occlusion. SPECT using Thalium-201(and Tc-99m) and triphenyl tetrazolium chloride (TTC) stain were performed after sacrifice. The quantity of TTC stain and amount of Thalium-201 uptake was compaired by image program, Vision workstation. Results: All pigs were survived during and after experiments. Two pigs demonstrated the ventricular tachycardia during experiments. The coronary angiography revealed total occlulded LAD persistently 4 weeks later in all pigs. The LV angiography showed akinetic movement in apicoanterior wall motion. The SPECT revealed perfusion defect in apicoanterior wall of the all pigs, regularly. The percent perfusion defect was 22.2짹3.06%. The TTC stain showed no uptake in the myocardium of apicoanterior wall. The percent non-stainable myocardium was 23.5짹2.70%. Conclusions: This pig model of acute myocardial infarction is reliable, reproducible, and amenable to investigate other medical and surgical therapies. Further study was warranted for chronic heart failure model using same method.