Background : The dilivery of human adipose-derived stem cells (hADSCs) for promoting tissue repair become a new therapeutic option. Previous study showed that mouse ADSCs transplantation in C57BL/6 mice model of AMI was promising results. Here, we would like to investigate the effects of hADSC and granulocyte chemotactic protein-2 (GCP-2), as proangiogenic cytokine, expression hADSCs (T-hADSCs) in NOD/SCID mice model 4 weeks after AMI induction.
Methods : 30 NOD/SCID mice were randomized into 3 groups, AMI+media (control, n=10), AMI+hADSCs (n=10) and AMI+T-hADSCs (n=10). AMI was produced by left anterior descending coronary artery ligations. After AMI induction, 1×106 hADSCs, T-hADSCs and media were injected intramyocardially. After 4 weeks, echocardiographic and histological analyses of surviving NOD/SCID mice (n=26) were conducted.
Results : The LVEFs in hADSCs and T-hADSCs groups were improved compared with control group, 68.7±8.0%, 72.3±7.8% and 48.1±5.0%, respectively (p<0.05). The LVEDD and LVESD in both hADSCs and T-hADSCs groups were improved than those of control group (p<0.05). Both hADSCs and T-hADSCs were observed to migrate and intergrate into injured myocarium and increase vascular density contrast to control. Microvascular density (MVD) of T-hADSCs group was significantly higher than that of both hADSCs and contol, 10.5±1.7/0.2㎟, 10.0±2.1/0.2㎟ and 8.5±1.3/0.2㎟, respectively (p<0.05).
Conclusions : In NOD/SCID 4 weeks after AMI models, transplantation of hADSCs and T-ADSCs showed improvements of cardiac function and ventricle remodeling than control group. Specially, GCP-2 as proangiogenic chemokine, seemed to enhance angiogenesis in repair of ischemic myocardium.
|
