Background: Currently, transthoracic real-time 3-D Echocardiography (RT-3DE) is limited by the need to gate acquisition over multiple cardiac cycles for full volume B-Mode and Color Flow Doppler (CFD) imaging. This limitation can be overcome by a system which is capable of broad beam transmission and receive up to 64 beams simultaneously. We assessed the clinical feasibility of a single heart-beat RT-3DE technique for transthoracic echocardiography. Methods: 20 patients were prospectively enrolled in this study. Single heart beat, full volume B-Mode and CFD at 15 vol/sec data were obtained using a 4Z1c matrix array transducer on the SC2000TM platform (Siemens, Mountain View, CA). This system also incorporates Coherent Volume Formation��� technology which allows 3-D imaging at high volume rates without loss of spatial information. Results: All data was acquired successfully for analysis. Fig. 1 shows B-Mode 90 x 90 image of a normal LV in which the enitre LV and RV is imaged within the volume. Fig. 2 shows contraction front mapping and displaying parameters of LV function derived from a fully-automated algorithm based on learned pattern recognition technology. Fig. 3 shows example of apical 4 chamber view in patient with heart failure. Fig. 4 shows CFD of mitral regurgitation in a single-heart beat within a 60 x 60 B-mode volume and 40 x 30 color volume. Conclusion: Non-stitched, single heart beat full volume B-Mode and CFD RT-3DE is feasible,and has the potential to facilitate clinical adoption and maybe more accurate than gated 3DE volumes.