Objectives; Although human adipose tissue-derived stem cells (hADSCs) were known to multi-potent cells, the adequate conditions for differentiation into the cardiomyocytes (CMCs) or endothelial cells (ECs), two major cells of cardiovascular system, are not elucidated. We investigated to adequate condition for differentiation of hADSCs into CMCs or ECs, especially according to the different culture media. Methods; hADSCs were isolated from abdominal adipose tissue and cultured in Minimum Essential Medium alpha (慣-MEM). hADSCs (second passage) were exposed to 5-azacytine (10µM/L) for 24 or 48 hours. At 1 or 2 weeks after 5-azacytine exposure, cardiac specific genes (GATA-4, NKx2.5, Troponin-I) were evaluated by RT-PCR. For adequate media condition and capacity of differentiation into CMCs or ECs, hADSCs were cultured under the 3 different media (慣-MEM, EGM-2; Endothelium Growth Media-2, CMC culture media; mixture of 慣-MEM and M-199) without 5-azacytidine for 1 or 2 weeks. Cardiac and endothelial specific genes and proteins were evaluated by RT-PCR and confocal microscopy respectively. Results: Before 5-azacytidine treatment, hADSCs under the 慣-MEM showed expressions of GATA-4, Nkx-2.5 and Troponin-I. However, Nkx-2.5 and Troponin-I were not expressed after 5-azacytidine treatment. hADSCs under the EGM-2 showed endothelial-specific genes and proteins (KDR and vWF). hADSCs under CMC culture media didn���t show Nkx2.5, GATA4 or Troponin-I by RT-PCR, but only Troponin-I was observed by confocal microscopy. Conclusion: Basal expression of cardiac specific genes in hADSCs under 慣-MEM without any treatment including 5-azacytidine suggests the feasibility of hADSCs for celluar cardiomyoplasty without special processing. And also, hADSCs under EGM-2 showed the potential of cytokine-dependent ECs differentiation. These findings suggest hADSC is one of the effective cell sources of cell therapy for angiogenesis and cardiomyoplasty.