Indocyanine Green (ICG) angiography has been usually used for imaging posterior part of the eye which contains choroidal vessels and superficial tissues such as transplanted skin graft. ICG possesses various advantages for in vivo imaging: distribution in the plasma with little extravasation; excretion into bile without biotransformation; and low incidence of adverse effects. ICG fluoresces in the near Infrared spectrum. Owing to low auto-fluorescence and low tissue scattering, near infrared is well-suited for imaging deep tissues. Taking advantage of near infrared and ICG, we tried to develop a novel method to visualize deep vessels and measure tissue perfusion. ICG Fluorescence Image (IFI) was obtained using CCD digital imaging system (Image station 4000MM, KODAK, NY, USA) after intravenous injection of 1.5 mg ICG/kg bodyweight into a tail vein of nude mice. We utilized a hind-limb ischemia model to test if IFI can represent tissue perfusion. Focal permanent ischemia was induced by femoral artery excision in nude mice (70 mice and 10 sham controls). Mice were classified into 2 groups, good and bad prognosis, according to degree of hindlimb necrosis at POD 7. Up to 40% of mice showed poor prognosis and 60% showed favorable prognosis, better than below-ankle necrosis at POD 7. Laser Doppler Image (LDI) and IFI were taken serially at POD 0, 1, 2, 3, and 4. LDI technique revealed decreased perfusion in ischemic hindlimb region, however, it could not differentiate good prognosis group from bad prognosis groups. IFI showed higher topographical resolution which shows diffuse (capillary topology) and discrete (artery and vein) patterns. We could make an estimate good or poor prognosis with IFIs which were taken at POD 0. But LDI cannot help to estimate sensitively tissue perfusion of ischemic hindlimb. We propose ICG fluorescence imaging method as a useful new method for detecting peripheral occlusive disorder as well as analyzing blood perfusion of deep tissues non-invasively.