Purpose: We evaluated urinary 8-iso-prostaglandin F 2α (8-iso-PGF 2α) and 8-hydroxydeoxyguanine (8-OHdG) as oxidative stress markers for the coronary atherosclerosis. Methods: We assessed urinary 8-iso-PGF 2α and 8-OHdG (OXIS International Inc, CA) levels by immunoassay in age-sex matched control (group1, n=43), minimal disease or normal looking coronary artery (group 2, n=60) in coronary angiography and presence of significant coronary artery disease (group 3, n=41) defined > 50% diameter stenosis of major coronary epicardial vessels. The patients with acute coronary syndrome, heart failure, renal failure and infectious condition were excluded. The association between tertile of 8-iso-PGF 2α or 8-OHdG and three groups was compared by chi-square test. Results: Baseline demography showed more prevalent of hypertension and diabetes in group 2 and 3. Urinary 8-iso-PGF 2α were 7.2 ± 9.1, 11.9 ± 12.3, and 18.4 ± 17.5 ng/mg creatinine (p= 0.001) and 8-OHdG were 1.2 ± 1.0, 1.8 ± 1.8, and 2.0 ± 1.8ng/mg creatinine (p = 0.033) for group 1, 2, and 3, respectively. There was significant association between the groups and tertile of urinary 8-iso-PGF 2α (group 1: 16.3%, group 2: 31.7%, and group 3: 51.2% in third tertile for 8-iso-PGF 2α , p <0.001), not in 8-OHdG (p = 0.055). Urinary 8-iso-PGF 2α levels were positively correlated with hsCRP (r = 0.23; p=0.008) and LDL cholesterol(r = 0.18; p=0.043). Conclusions: Urinary 8-iso-prostaglandin F 2α might be more reliable and useful oxidative stress marker than 8-hydroxydeoxyguanine in coronary atherosclerosis.
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