Introduction and Purpose: Bone marrow (BM) stromal cells are capable of differentiating into many cell lineages. During the developmental process, embryonal cells are exposed to blood flow generated by the beating heart. We assessed the hypothesis that shear stress modulate the differentiation of cardiomyocyte and endothelial cell from BM stromal cell. Methods and Results: BM mononuclear cell of C57/BL6 mouse were isolated and cultured. Three passage of stromal cells were exposed to shear stress by oscillatory, unidirectional laminar shear stress by cone-and- plate device at 10 dynes/cm2 for 18 hours. After shear stress, cell were cultured for seven days until analysis. RT-PCR and immunocytochemistry were performed to determine the endothelial-specific and cardiomyocyte-specific markers. Shear stress promotes BM stromal cell density and became elongated and aligned cell morphology. Cell cycle analysis showed shear stress increased the S, G2 and M phase from 30 % to 42 % (p<0.01). Shear stress accelerated the increase in the expression of angiopoietin-1, Flk-1, Flt-1, VE-caderin, PECAM-1, vWF, KDR, eNOS and CD31 as endothelial cell marker by RT-PCR and immunocytochemistry. The total length of tube was significantly greater in shear stressed BM stromal cell in Matrigel gel (static state vs. shear stress: 5 mm/cm2 vs. 300 mm/cm2; p<0.01). The expression of Wnt 11, Erg and BMP4 were increased in shear stressed cells. Shear stress promotes the expression of cardiomyocyte lineage marker such as carveolin 3, troponin T, alpha-cardiac actin and GATA-4 in the presence of adult mouse cardiomyocyte extract (100mcg/ml) compared with only cadiomyocyte extract treatment. Conclusions: Shear stress accerelated to induce BM stromal cell to cardiomyocyte in the presence of cardiomyocyte extract and endothelial cell.